Ischiocavernosus muscles are located on either side of the base of the penis in males and the clitoris in females. They extend from the ischial tuberosity, which is the bony prominence at the back of the pelvis, to the surrounding erectile tissue. In males, these muscles contribute to the pelvic floor and are positioned beneath the perineal membrane, anchoring to the ischium, and extending towards the midline to surround the corpus cavernosum. In females, their positioning is similar, providing support to the clitoris and assisting in its functional dynamics during arousal. The anatomical relationship of the ischiocavernosus to surrounding structures is essential for its proper function and impact on sexual health.